During this course you will learn the basic techniques of real-time PCR-analysis for the detection of viruses, bacteria and nematodes on potato tuber material. You will learn to carry out PCR- screening with the corresponding internal controls and gap and error analysis. The course contains a practical part and a theoretical part.

Duration of course 3 days
Course date at request
Course structure theory and practice
Location NAK, Emmeloord, The Netherlands
Instructor Mrs. L. Smits
Investment € 1.500,- per student (lunch and course folder are included)
Maximum group size 6 – 8 students

Topics which will be addressed

The theoretical part of the course takes one day and the practical part takes two days to complete. After completing the course you will receive a certificate of participation.

Theory:

  • Theory of pathogens (viruses ,bacteria and nematodes ) in potatoes
  • Theory behind the real-time multiplex PCR-technique, Elisa and IF
  • Quality aspects of PCR
  • Troubleshooting
  • Labvalidation
  • Use of robots

Practice:

  • Running a complete PCR-screening, by hand and robotized
  • Handling samples, analyzing data and performing quality controls.

Results after completing the course

After completing this course, you have gained more insight in the performance of a PCR-analysis of potato tuber material with the corresponding controls and analysis.

Ask for more information and let us know that you are interested this course by filling in the form below.