The NAK has a reliable and independent laboratory. The seed laboratory is certified by ISTA (International Seed Testing Association)-standards.

We offer the following laboratory test:

  • (seed)potatotests on viruses, nematodes and bacteria
  • cereals- and grasses-tests on germination, purity, moisture and health
  • soil sample-tests on nematodes

2020/2021

As a result of the measures we need to take to prevent COVID-19 infections, the capacity of potato tuber testing using the PCR method is this year lower than usual. This means that our available capacity is mainly needed to carry out legally required testing. Other requests for testing are, based on current expectations, only possible in August or from December. We will keep you informed via our website.

There is also limited capacity for carrying out potato tuber research using the Elisa method. Testing is  only possible from 15 December.

Please note that all samples that arrive without pre-requested labels and agreements on delivery dates cannot be processed.

You can request labels with the application form on our website.

Subjects

Virus and bacterial diagnostics in potatoes

The NAK can analyse your potato tubers on virus, bacteria and nematodes in combination or separately. It is not possible to offer us samples of potatoes from non-EU countries for research. This is a result of changed regulations and requirements that our laboratory must meet.

Test your potato tubers at the NAK and get the best insight in the quality of your potatoes

  • We give you the best insights into the quality of your potatoes by examining your tubers on several viruses and other diseases with the most advanced techniques.
  • Our specialized and qualified analysts will work on your product which guarantees the best results in predicting the disease status of your potatoes.
    Follow the steps in the next image to find out what is the best test for your tubers:

Click on the image to enlarge

  1. With PCR a combination test of all mentioned diseases is possible. But It is also possible to choose an examination on less diseases or only one.
  2. 4 x 50 is the standard merge for virus and Erwinia, other merges are possible (see price list). With a combination test the merge of the virus and Erwinia test needs to be the same.

For more information please contact our Customer service department: 0031 (0)527-635350 or nak@nak.nl

Available tests

Virus
We analyse potato tubers on different viruses with the ELISA- or PCR-technique.
Season 2020: it is possible to send in ELISA samples after the 15th of December, with a 25% addition on the costs.

Virus examination ELISA

Only available in the period of 15 August – 15 December

PCR
Determined potato virus PVY, PVA, PVS/PVX*, PLRV(potato leaf roll virus), PVV, PVM, PMTV en TSWV (Tomato spotted wilt virus).* PVX can be examined separately from PVS, but can also be combined in one reaction. Combination is cheaper since costs are only charged for one virus. Standard: PVY, PVA, PVX and PLRV (potato leaf roll virus)Max. 4 virus combinations in al PCR-test are possible. Testing on PVS is optional.

TRV (tobacco rattle virus), PMTV (potato mop top virus) can also be tested.

Required time for examination after receipt 6 – 9 weeks.The required time for examination depends on the weather conditions because the samples grow in a greenhouse. ≤ 2 weeks (priority ≤1 week)The PCR test is done directly on tuber material, which saves time compared to the ELISA test.
Packaging Mesh bag, close with a ty-rap. Mesh bag, close with a ty-rap.

In case of a combination test with M. chitwoodi/fallax and/or brownrot/ringrot, the sample should be delivered in a polyprop bag.

Sample size and merging ≤ 110 tubers, merged per 4 leaves
Explanation: A tuber grows to a plant, from each plant 1 leave will be picked. 4 leaves will be combined in 1 reaction, which is positive or negative for the tested organism. Per sample max. 96 plants will be tested, this gives a max. of 24 reactions per sample. Amount of plants that can be tested depends on their emergence in the greenhouse.Single tuber test (no merge) is optional.
5 options per sample:* 200 tubers, 4 reactions (4 x 50 tub)

* 200 tubers, 10 reactions (10 x 20 tub)

* 100 tubers, 10 reactions (10 x 10 tub)

* 50 tubers, 1 reaction (1 x 50 tub)

* 20 tubers, 1 reaction (1 x 20 tub)

Explanation (for example 4×50): you deliver a sample of 200 tubers, our laboratory divides them in 4 sub-samples of 50 tubers each. Every subsample gives a reaction, the reaction is positive or negative for the tested organism.

Erwinia: bacterial blackleg/stem rot
We examine potato tubers on the presence of bacteria which causes blackleg/stem rot. This test will detect (latent) infections of Pectobacterium atrosepticum (Pa), Dickeya spp. (Dic), Pectobacterium parmentieri (Pp) and Pectobacterium brasiliense (Pb).

Erwinia
Examination method PCR
Required time for examination ≤ 3 weeks (priority ≤ 2 weeks)
Packaging Mesh bag, close with a ty-rap.In case of a combination test with M. chitwoodi/fallax and/or browrot/ringrot, the sample should be delivered in a polyprop bag.
Sample size and merging 5 options per sample:* 200 tubers, 4 reactions (4 x 50 tubers)

* 200 tubers, 10 reactions (10 x 20 tubers)

* 100 tubers, 10 reactions (10 x 10 tubers)

* 50 tubers, 1 reaction (1 x 50 tubers)

* 20 tubers, 1 reaction (1 x 20 tubers)

Explanation (for example 4×50): you deliver a sample of 200 tubers, our laboratory divides them in 4 sub-samples of 50 tubers each. Every subsample gives a reaction, the reaction is positive or negative for the tested organism.

Meloidogyne chitwoodi and fallax
Meloidogyne chitwoodi and Meloidogyne fallax are both quarantine nematodes that can cause severe damage to their host plants, of which Solanum tuberosum (potato) is the most important.

Meloidogyne chitwoodi and fallax
Examination method PCR
Required time for examination ≤ 3 weeks (priority ≤ 2 weeks)
Packaging Polyprop bag,

close with a ty-rap.

Sample size and merging 200 tubers,

100 tubers merged in 1 reaction (2 reactions per sample)

Brownrot and Ringrot
We offer tests in which we can detect (latent) infections of bacterial ring rot caused by Clavibacter michiganensis subsp. sepedonicus and/or brownrot caused by Ralstonia solanacearum.
Please be aware that if the sample is positive for brownrot or ringrot the NAK has the obligation to report this to the Dutch NPPO.

Ringrot and brownrot examination
Examination method IF-test
Required time for examination ≤ 2 weeks (priority ≤ 1 week)

When a sample gives a positive reaction in the IF-test, the sample will be send to the Dutch NPPO where several confirmation tests will be carried out. The complete diagnosis of a positive sample will be reported to you between 2 and 15 weeks after you received the result of the IF-test.

Packaging Polyprop bag,

close with a ty-rap.

Sample size and merging 200 tubers,

200 potato tubers merged in 1 ‘reaction’.

Is your desired test not mentioned above or would you like an price offer? Please send an email with a clear description to nak@nak.nl.

Combination of tests

We advise you to combine various tests on the same potato sample of 200 tubers. It reduces costs.
All of the following tests can be performed on one single potato sample of 200 tubers:

  • Virus PCR (standard combination PVY, PVA, PVX and PLRV)
  • Erwinia (blackleg tests)
  • Meloidogyne chitwoodi and M. fallax
  • Brownrot and ringrot.

Test results

You will receive the results per e-mail as an Pdf-file (Excel-file is also possible).
We present the number of positive (‘diseased’) reactions instead of disease percentage and spread. We have 2 documents for you if you want the percentage of diseased plants and spread:

Sample labelling

  • All your samples should be labelled with unique NAK sample labels. You can order sample labels with the request form.  Please send your request form to nak@nak.nl. The sample labels will be send to you by mail.
  • A sample label consists of 2 parts. You should attach one part on the outside of the sample bag and one inside the sample bag. On the side of each label-part is mentioned where it should be used (in = inside the bag, aan = outside the bag).
  • The samples with GMO modified material should be marked with special GMO labels. Contact us for permission via nak@nak.nl, before sending GMO samples.

Packaging and logistics

Samples are only accepted on weekdays from 8.00 – 17.00 hour (except on Dutch public holidays). Please inform us 3 working days before sending your samples via nak@nak.nl with the following details:

  1. expected date of delivery
  2. the amount of samples
  3. description which tests needs to be performed on your samples.

Delivery address: NAK, Randweg 14, 8304 AS Emmeloord, The Netherlands.

Advice and support

Specific questions regarding tests, samples, analytical issues and logistics can be forwarded to the
Customer service department via 0031 (0)527-635350 or nak@nak.nl
The NAK standard terms and conditions for the supply and services apply to our activities.

Costs

See the pricelist